Live dead sperm ratio

Finally, it was determined that a second population of dead sperm as reported in the literature was in fact a technical artefact caused by killing of sperm with freeze—thawing. A gating strategy was developed for the separation of populations of live and dead cells in honeybee sperm samples. Sperm viability, in particular, has become a key measure for semen quality in recent years, and often refers to the proportion of live sperm in an ejaculate. This dual fluorescent stain can be used as an objective evaluation of live: Finally, a technique preferred by many for evaluating sperm morphology is to use no stain at all, but to visualize sperm under differential interference contrast microscopy. Monitoring adherent cell cultures in microtiterplates by a wireless sensory system. The problem can involve the head, midpiece or tail.

PNA is a peanut derivative that will actively bind to the sperm acrosome the part of the sperm responsible for binding to and fertilising the egg. What type of cell is parabasal , intermediate, superficial, cornified cells? Another stain that can be used, and is demonstrated below, is toluidine blue.